Cellquest Software -

is a software application developed by Becton Dickinson (BD Biosciences) for the acquisition, analysis, and display of flow cytometry data. For nearly two decades, it was the industry-standard program for BD FACScan, FACSort, FACSCalibur, and FACSCanto instruments.

One of the most critical features introduced and refined in CellQuest was . cellquest software

| Feature | CellQuest (Legacy) | FACSDiva (Modern) | | :--- | :--- | :--- | | | Primarily FACSCalibur | FACSCanto, LSRFortessa, etc. | | Interface | 2D, menu-heavy | 3D visualization, tabbed interface | | Compensation | Manual slider adjustment | Automated calculation wizards | | OS Support | Mac OS Classic/Early OS X | Windows 10/11, Modern Linux | | Experiment Layout | Manual setup per run | Hierarchy-based experiment templates | is a software application developed by Becton Dickinson

For decades, CellQuest served as the digital backbone for classic cellular analysis instruments. It integrated physical fluidics and optics systems directly into an interactive desktop interface. It has been utilized extensively in legacy setups for critical research workflows including: | Feature | CellQuest (Legacy) | FACSDiva (Modern)

Perhaps the most enduring legacy of CellQuest is its adherence to the file format. Files acquired in CellQuest 20 years ago can still be opened in modern analysis software like FlowJo, FCS Express, or Cytobank. This interoperability is vital for longitudinal clinical studies.