| Concentration (M) | Absorbance | | --- | --- | | 0.1 | 0.2 | | 0.2 | 0.4 | | 0.5 | 0.8 | | 1.0 | 1.2 |
Prism’s built-in dose-response models expect .
Click Analyze and select Interpolate a Standard Curve . For many biological assays, a Sigmoidal, 4PL (four-parameter logistic) model is the industry standard.
Once you have your experimental results (e.g., absorbance from an ELISA or radioactivity from a binding assay), you can use the GraphPad Prism desktop software to turn those values back into molarity units. Step 1: Interpolating from a Standard Curve
Output: P-value for difference in EC50, shift in curve.