Today, we’re going to map out the and discuss how to leverage them for seamless construct assembly.
Never assume a site is unique—always run a virtual digest using SnapGene, Benchling, or NEBcutter before ordering primers. pgps3 unique restriction sites
The vector is a specialized 4,293 bp plasmid primarily utilized as a "Transprimer" donor within the GPS-M Mutagenesis System . Developed by New England Biolabs (NEB) , this vector is critical for in vitro random mutagenesis, where a mobile genetic element (the Transprimer) is moved from the donor plasmid into a target DNA molecule via TnsABC transposase. Today, we’re going to map out the and
) into the pGPS3 vector to create custom transposon constructs. pgps3 unique restriction sites
For seamless assembly (no restriction ligation), cut PGPS3 with a like PstI or NotI . Then: