: Use a sample with 0% glucose (typically distilled water or assay buffer) to calibrate the instrument to zero absorbance. 2. Experimental Measurement
Even a tiny drop of extra liquid can throw off the concentration of a standard. glucose standard curve
Mira looked at the graph on the screen. It was just a straight line on a piece of paper, but looking at it, she realized it was a bridge. They had walked from the known world of pure standards across the bridge of mathematics to measure the unknown world of the cave. : Use a sample with 0% glucose (typically
The R² value, or coefficient of determination, tells you how reliable your curve is. A value of 0.99 or higher is the gold standard, indicating that your dilutions were accurate and the machine is calibrated correctly. Why It Matters Clinical Diagnostics Mira looked at the graph on the screen